elisa for high molecular weight (hmw) adiponectin  (ALPCO)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Immunomodulatory Role of an Ayurvedic Formulation on Imbalanced Immunometabolics during Inflammatory Responses of Obesity and Prediabetic Disease

doi: 10.1155/2013/795072

Figure Lengend Snippet: Kal-1 rectifies the hormonal imbalance in mice fed on high-fat high-sugar diets. (a) Effect of Kal-1 on resistin levels in high-fat high-sugar fed mice at week 15. (b) Effect of Kal-1 on leptin levels in high-fat high-sugar fed mice at week 15. (c) Effect of Kal-1 on high-molecular-weight adiponectin levels in high-fat high-sugar fed mice at week 15. All doses (5, 20, and 75 μ L) of Kal-1 were supplemented along with HFHSD. LF: low fat control, HF: high-fat high-sugar control. All the values represent mean ± SEM from five animals.

Article Snippet: Leptin and resistin levels were measured using commercially available radioimmunoassay kits (Quantikine, mouse leptin and mouse resistin, immunoassay, R&D, Minneapolis, MN, USA) and high-molecular-weight adiponectin (ALPCO, adiponectin mouse total, HMW, Salem, NH, USA).

Techniques: Molecular Weight

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Immunomodulatory Role of an Ayurvedic Formulation on Imbalanced Immunometabolics during Inflammatory Responses of Obesity and Prediabetic Disease

doi: 10.1155/2013/795072

Figure Lengend Snippet: Treatment of Kal-1 brings back the levels of pro- and antihormonal levels to normal which were showing metabolic disturbed contour in high-fat high-sugar fed mice at weeks 22, 26, and 30. (a) Effect of Kal-1 treatment on leptin profile in high-fat high-sugar diet fed mice at weeks 22, 26, and 30. (b) Effect of Kal-1 treatment on high-molecular-weight adiponectin profile in high-fat high-sugar diet fed mice at weeks 22, 26, and 30. Treatment with all doses (5, 20, and 75 μ L) of Kal-1 was started only after 21 weeks (induction period) along with high-fat high-sugar diet. LF: low-fat control, HF: high-fat high-sugar control. All the values represent mean ± SEM from five animals.

Article Snippet: Leptin and resistin levels were measured using commercially available radioimmunoassay kits (Quantikine, mouse leptin and mouse resistin, immunoassay, R&D, Minneapolis, MN, USA) and high-molecular-weight adiponectin (ALPCO, adiponectin mouse total, HMW, Salem, NH, USA).

Techniques: Molecular Weight

Journal: Molecular Metabolism

Article Title: Adipocyte-specific ablation of the Ca 2+ pump SERCA2 impairs whole-body metabolic function and reveals the diverse metabolic flexibility of white and brown adipose tissue

doi: 10.1016/j.molmet.2022.101535

Figure Lengend Snippet:

Article Snippet: High molecular weight (HMW) adiponectin ELISA kit , MyBiosource , MBS028367.

Techniques: Recombinant, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Reverse Transcription, Enzyme-linked Immunosorbent Assay, High Molecular Weight, Gene Expression, Knock-In, Quantitative RT-PCR, Software